cfx96 real time pcr instrument  (Bio-Rad)

Journal: Biology

Article Title: One-Pot LAMP-Coupled CRISPR/Cas12b Assay Enables Sensitive Detection of Helicobacter pylori

doi: 10.3390/biology15100797

Figure Lengend Snippet: LAMP Primer Panel Screening. ( a ) Amplification curves for real-time LAMP; ( b ) agarose gel electrophoresis. CagA2/4/7: Positive controls for the 2nd, 4th, and 7th sets of CagA LAMP primers, respectively; NTC-CagA2/4/7: Negative controls for the 2nd, 4th, and 7th sets of CagA LAMP primers, respectively.

Article Snippet: The key instruments applied in this investigation included a −80 °C ultra-low temperature freezer (Aucma, Qingdao, China), a high-speed microcentrifuge (Michael Laboratory Instrument, Changsha, China), a microspectrophotometer (Xinling Biotechnology, Shanghai, China), micropipettes (Eppendorf, Hamburg, Germany), a biosafety cabinet (Xinbeixi Biotechnology, Jinan, China), Real-time fluorescence quantitative PCR(qPCR) instrument (Bioer Technology, Hangzhou, China), an electronic balance (Puchun Instrument, Shanghai, China), an electrophoresis apparatus (Yixin Analytical Instrument, Shanghai, China), and a gel imaging system (Yixin Analytical Instrument, Shanghai, China).

Techniques: Amplification, Agarose Gel Electrophoresis

Journal: Applied and Environmental Microbiology

Article Title: Continuous recirculation of hydroponic-nutrient solutions shifts bacterial communities and induces plant-defense gene expression in lettuce

doi: 10.1128/aem.01647-25

Figure Lengend Snippet: P. myriotylum detected in ( a ) nutrient solutions and lettuce cv. Rex ( b ) roots grown in autoclaved or non-autoclaved nutrient solution inoculated with P. myriotylum (+ P ) used continuously for five growing cycles. Genomic DNA of P. myriotylum was quantified using real-time quantitative PCR. Means with the same letters are not significantly different according to Tukey’s HSD test at α = 0.05 ( n = 5). Statistical comparisons reflect the interaction among all treatment combinations of nutrient solution type, P. myriotylum inoculation, and recirculation cycle (nutrient solution: P = 0.6137, root: P = <0.0001).

Article Snippet: RT-qPCR was utilized with each gene in the CFX Connect Real-Time PCR Detection System instrument (BioRad, CA, USA).

Techniques: Real-time Polymerase Chain Reaction